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Characterization of the self-targeting Type IV CRISPR interference system in Pseudomonas oleovorans - Nature Microbiology

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Characterization of the self-targeting Type IV CRISPR interference system in Pseudomonas oleovorans - Nature Microbiology

Bacterial Type IV CRISPR-Cas systems are thought to rely on multi-subunit ribonucleoprotein complexes to interfere with mobile genetic elements, but the substrate requirements and potential DNA nuclease activities for many systems within this type are uncharacterized. Here we show that the native Pseudomonas oleovorans Type IV-A CRISPR-Cas system targets DNA in a PAM-dependent manner and elicits interference without showing DNA nuclease activity.

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Characterization of the self-targeting Type IV CRISPR interference system in Pseudomonas oleovorans - Nature Microbiology

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Characterization of the self-targeting Type IV CRISPR interference system in Pseudomonas oleovorans - Nature Microbiology

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