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A brilliant monomeric red fluorescent protein combining high brightness and fast maturation - Nature Methods

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A brilliant monomeric red fluorescent protein combining high brightness and fast maturation - Nature Methods

We evolved the brilliant monomeric red fluorescent protein mScarlet3 using a multiparameter screening approach. Owing to a newly engineered hydrophobic patch inside its β-barrel structure, mScarlet3 combines a high quantum yield and high fluorescence lifetime with fast and complete maturation. Consequently, mScarlet3 performs well as a fusion tag in live-cell imaging.

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A brilliant monomeric red fluorescent protein combining high brightness and fast maturation - Nature Methods

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A brilliant monomeric red fluorescent protein combining high brightness and fast maturation - Nature Methods

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