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Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity - Nature Biotechnology

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Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity - Nature Biotechnology

Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination method reduces photobleaching effects 1.5–9.2-fold, can be easily implemented on commercial microscopes and is effective in eukaryotic and prokaryotic cells with a wide range of fluorescent proteins.

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Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity - Nature Biotechnology

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Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity - Nature Biotechnology

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