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The eINTACT method for studying nuclear changes in host plant cells targeted by bacterial effectors in native infection contexts - Nature Protocols
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Type-III effector proteins are major virulence determinants that most gram-negative bacteria inject into host cells to manipulate cellular processes for infection. Because effector-targeted cells are embedded and underrepresented in infected plant tissues, it is technically challenging to isolate them for focused studies of effector-induced cellular changes.
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