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Spatiotemporal, optogenetic control of gene expression in organoids - Nature Methods

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Spatiotemporal, optogenetic control of gene expression in organoids - Nature Methods

Organoids derived from stem cells have become an increasingly important tool for studying human development and modeling disease. However, methods are still needed to control and study spatiotemporal patterns of gene expression in organoids. Here we combined optogenetics and gene perturbation technologies to activate or knock-down RNA of target genes in programmable spatiotemporal patterns.

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Spatiotemporal, optogenetic control of gene expression in organoids - Nature Methods

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Spatiotemporal, optogenetic control of gene expression in organoids - Nature Methods

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