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Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides - Nature Chemical Biology

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Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides - Nature Chemical Biology

The modular nature of nonribosomal peptide biosynthesis has driven efforts to generate peptide analogs by substituting amino acid-specifying domains within nonribosomal peptide synthetase (NRPS) enzymes. Rational NRPS engineering has increasingly focused on finding evolutionarily favored recombination sites for domain substitution. Here we present an alternative evolution-inspired approach that involves large-scale diversification and screening.

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Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides - Nature Chemical Biology

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Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides - Nature Chemical Biology

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