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Coordination of cohesin and DNA replication observed with purified proteins - Nature

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Coordination of cohesin and DNA replication observed with purified proteins - Nature

Two newly duplicated copies of genomic DNA are held together by the ring-shaped cohesin complex to ensure faithful inheritance of the genome during cell division1–3. Cohesin mediates sister chromatid cohesion by topologically entrapping two sister DNAs during DNA replication4,5, but how cohesion is established at the replication fork is poorly understood. Here, we studied the interplay between cohesin and replication by reconstituting a functional replisome using purified proteins.

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Coordination of cohesin and DNA replication observed with purified proteins - Nature

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Coordination of cohesin and DNA replication observed with purified proteins - Nature

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