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Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters - Nature Methods

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Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters - Nature Methods

The inability to scalably and precisely measure the activity of developmental cis-regulatory elements (CREs) in multicellular systems is a bottleneck in genomics. Here we develop a dual RNA cassette that decouples the detection and quantification tasks inherent to multiplex single-cell reporter assays. The resulting measurement of reporter expression is accurate over multiple orders of magnitude, with a precision approaching the limit set by Poisson counting noise.

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Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters - Nature Methods

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Multiplex profiling of developmental cis-regulatory elements with quantitative single-cell expression reporters - Nature Methods

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